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. 2024 May 30;27(8):1555–1564. doi: 10.1038/s41593-024-01654-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, Left: experimental design. CW, complex wheel. Middle: immunohistochemistry of PDGFRα (green) revealed focal OPC hyperdensities (HD) in the Nf1^OPC-Het (Nf1^+/fl;Pdgfra::Cre) mice. Scale bar, 1 mm. Right: representative non-HD (blue outline) and HD (yellow outline) OPCs (PDGFRα, green). Scale bar, 20 µm. b, PDGFRα immunohistochemistry (green) of Nf1^+/− mouse brains at P35, P84 and P168. Inset, two focal OPC hyperdensities and the surrounding nonhyperdensity regions. Scale bar, 1 mm. c, Quantification of b revealed an increased area of focal OPC hyperdensities in Nf1^+/− mouse brains at P35, P84 and P168. **P = 0.0092 (P35 versus P84), 0.0089 (P84 versus P168). N = 3, 9 and 7 (left to right). d, Quantification of OPC (PDGFRα⁺ cells) density in P35 mice revealed increased OPC density within the hyperdensity (HD) areas, relative to nonhyperdensity (non-HD) areas, in Nf1^+/− mouse brains and WT mouse brains. N = 6 per group. ****P < 0.001. e, Quantification of OL (ASPA⁺ cells) density in the cingulum of P184 Nf1^+/− mice revealed no differences between hyperdensities (HD) and nonhyperdensity (non-HD) areas. N = 3 per group. Unpaired t-test with Welch’s correction. f, Immunohistochemistry of PDGFRα (green) revealed focal OPC hyperdensities in the brains of Kras^LSL-G12D/+;Olig2::Cre mice. Scale bar, 200 µm. Brown–Forsythe ANOVA test with Dunnett’s T3 multiple comparisons (c, F = 20.57; d, F = 216). Data shown as mean ± s.e.m.; each point represents one mouse (c–e); two-sided; NS, not significant (P > 0.05).

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