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. 2024 May 30;27(8):1555–1564. doi: 10.1038/s41593-024-01654-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a,b, WT, Nf1^+/− and Nf1^−/− OPCs were cultured and induced for differentiation. Immunocytochemistry using MBP (oligodendrocyte marker) and Olig2 (oligodendroglial lineage marker) antibodies revealed a reduced percentage of oligodendrocytes (the number of MBP⁺ cells divided by the number of Olig2⁺ cells) in Nf1^+/− and Nf1^−/− OPCs, compared to the WT OPCs. Brown–Forsythe ANOVA test (F = 18.15) with Dunnett’s T3 multiple comparisons. *P = 0.0111; ***P = 0.0008. Scale bar, 50 µm. c, Immunohistochemistry performed in the cingulum of 1-month-old Nf1^WT and Nf1^OPC-iKO mice (tamoxifen injection at P28) revealed that Nf1^OPC-iKO mice had reduced density of oligodendrocytes (Olig2⁺/PDGFRα⁻ cells). N = 4 per group. *P = 0.0382. d, Immunohistochemistry in the cingulum of 4-month-old Nf1^WT (N = 7) and Nf1^OPC-iKO (N = 5) mice revealed similar oligodendrocyte (ASPA⁺ cells) density. Unpaired t-test with Welch’s correction (c and d). Data shown as mean ± s.e.m.; each point represents one mouse (c and d); two-sided; NS, not significant (P > 0.05).

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