Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 May 30;27(8):1555–1564. doi: 10.1038/s41593-024-01654-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 1 — (a) Immunohistochemistry revealed no change in the density of new oligodendrocytes (EdU⁺/ASPA⁺) between the ipsilateral (blue, ipsi) and the contralateral (white, contra) side, during the 3-hour of the optogenetics experiment. Unpaired t test with Welch’s correction; N = 4 in each group. (b-d) Immunohistochemistry of the cingulum revealed no change in the density of microglia (Iba1⁺ cells) or reactive microglia (CD68⁺/Iba1⁺ cells) between the ipsilateral (blue, ipsi) and the contralateral (white, contra) side of Nf1^WT (WT, N = 4), Nf1^OPC-iHet (Nf1^+/-, N = 4) and Nf1^OPC-iKO (Nf1^-/-, N = 3) mice. Paired t test. (e) Cleaved caspase-3 (CC3) immunohistochemistry in the positive control (cuprizone-induced demyelination) showed staining of CC3⁺ (green) cells. Scale bar, 100 µm. (f) Immunohistochemistry of the cingulum from Nf1^WT and Nf1^OPC-iKO mice exposed to optogenetic stimulation revealed few CC3⁺ cells. Scale bar, 100 µm. (g) Nf1^WT mice infected with AAV carrying eYFP did not show changes in the density of new OPCs (EdU⁺/PDGFRα⁺) on the ipsilateral (blue, ipsi) side, relative to the contralateral (white, contra) side, of the brain. Mann-Whitney test; N = 3 in each group. (h) The density of total OPCs (PDGFRα⁺) in the contralateral side of optogenetically manipulated Nf1^OPC-iKO (N = 5), but not Nf1^OPC-iHet (N = 4), mice is increased relative to the Nf1^WT (N = 7) controls. Brown-Forsythe ANOVA test (F = 34.93) with Dunnett’s T3 multiple comparisons. ***, P = 0.0009. Data shown as mean ± SEM; each point = one mouse (a,b,c,d,g,h); two-sided; ns, not significant (P > 0.05).

Source data