Extended Data Fig. 3. The focal OPC hyperdensities in the Nf1+/- mouse brain lack obvious changes in other glial cells, senescence, or apoptosis.
(a, b) Immunohistochemistry of Nf1+/- mouse brains revealed no changes in the density of microglia (Iba1+ cells) or reactive microglia (CD68+/Iba1+ cells) in regions with focal OPC hyperdensities (HD) relative to those without (non-HD). Wilcoxon test (grey matter Iba1+ cells); Paired t test (other comparisons); Scale bars, 50 µm. N = 4 per group. (c) RNAscope revealed no changes in reactive astrocytes (Cxcl10+/Sox9+ cells) in areas with focal OPC hyperdensities relative to those without. N = 5 per group. Paired t test; Scale bar, 50 µm. (d) Immunohistochemistry revealed no senescent (p21+) or apoptotic (TUNEL+) cells in areas with OPC hyperdensities (HD), relative to those without (non-HD). Scale bar, 20 µm. Data are shown as mean ± SEM; each point = one mouse (b,c); two-sided; ns, not significant (P > 0.05).