Extended Data Fig. 3. Effect of CD8+ depletion on behavior and on amyloid plaque load in 5xFAD mice.
a, Representative image and quantification showing number of CD8+ T cells in mice (7.5 month-old) treated with anti-CD8 and isotype control antibodies. Each dot (red) represents one CD8+ T cell. Scale bar, 500 µm. Statistical significance was determined for n = 9–10 animals using unpaired two-sided Student’s t-test (control, anti-CD8, ***p = 0.001). b, Representative image showing Aβ plaque load (green) in mice (7.5 month-old) treated with anti-CD8 and isotype control antibodies. Scale bar, 500 µm. Quantifications indicate proportion of Aβ positive area normalized to total area in the cortex. Statistical significance was determined for n = 9 animals using unpaired Student’s t-test. c, Representative image and quantification indicating number of BCAS1+ (green) swellings lacking a nucleus (stained with DAPI) in the cortex of 7.5 month-old 5xFAD mice treated with anti-CD8 and isotype control antibodies. Scale bar, 10 µm. Statistical significance was determined for n = 5 animals using unpaired two-sided Student’s t-test (control, anti-CD8, *p = 0.038). d, Behavior of 7.5 month-old WT and 5xFAD mice following 6-week treatment with anti-CD8 and isotype control antibodies in an open-field test. Quantifications indicate fecal boli count, total distance travelled, frequency to enter the center and cumulative time spent in the center. Statistical significance was determined for n = 9-10 animals by one-way ANOVA with Tukey’s post hoc test. (Fecal boli count, WT, 5xFAD+control, **p = 0.0017; Distance traveled, WT, 5xFAD+control,*p = 0.03; Center enter frequency, 5xFAD+control, 5xFAD+anti-CD8, *p = 0.02; Cumulative duration in center, 5xFAD+control, 5xFAD+anti-CD8, *p = 0.01). e, Behavior of 7.5 month-old mice following 6-week treatment with anti-CD8 and isotype control antibodies in an elevated plus maze test. Quantifications indicate fecal boli count, total distance travelled, total time spent in the open arms and total time spent in the closed arms. Statistical significance was determined for n = 9-10 animals by one-way ANOVA with Tukey’s post hoc test. (Fecal boli count, WT, 5xFAD+control, *p = 0.03; Open arm duration, WT, 5xFAD+control, *p = 0.03; Open arm duration, WT, 5xFAD+anti-CD8, ***p = 0.0001; Close arm duration, WT, 5xFAD+anti-CD8, *p = 0.02). Each point on the graph represents one animal. Data is presented as mean ± SEM.