Figure 1.

Clustering of snRNA-seq data. Single nucleus RNA sequencing was performed on nucleus accumbens samples from male, drug-naïve Brown Norway rats and data were clustered based on transcriptomic profile. (a) Uniform manifold approximation and projection (UMAP) dimension reduction plot with nuclei colored by major cell type. Normalized expression of two MSN marker genes (Bcl11b and Pde10a) is visualized by both violin plot (b) and scatter plot (c). (d–k) Feature plots display expression of markers for major glial populations and non-MSN neuronal subtypes for cluster identification.