Fig. 2.

GzmB promotes vascular sprouting and VEGF-A expression while reducing TSP-1 in the RPE-choroid. A Representative images show the effects of exogenous GzmB treatment compared with controls at Day 8 of explant culture. Yellow lines around sprouting area show maximum extent of sprouting. B Quantification of vascular sprouting at Day 8 of CSA. Choroid sprouting is significantly increased with GzmB treatment compared with control (PBS) treatment. C–E Western blot and MesoScale Discovery (MSD) multiplex assays reveal GzmB-induced increased expression of VEGF-A in CSA supernatant. C Representative western blot of VEGF-A and its total protein control blot for total protein normalization (iBright Imaging Systems) D Densitometric quantification of VEGF-A in western blot. E Quantification of VEGF-A in MSD multiplex assays. F–G Western blot assays reveal GzmB-induced proteolysis of TSP-1 in CSA supernatant. The total protein control blot is shown in Supplementary Fig. 1. F Representative western blot of TSP-1. G Densitometric quantification of TSP-1 western blot. Results are presented as mean ± SEM. *p < 0.05 in T-test. n of A–D, F and G = 6 per group, n of E = 4–6 per group