FIG. 3.

Processing products derived from domains P1-2A and 2BC of the HAV polyprotein mutated at the 3A/3B and/or 3B/3C site. The complete HAV genomes of wild-type (wt) and mutated sequence (mutants 1 to 6) was expressed in COS-7 cells. Equal amounts of cells extract were analyzed by immunoblotting with anti-VP1, anti-VP0, anti-VP3, anti-2C3A, and anti-2B antibodies. The asterisk indicates an unidentified host protein immunodetected by anti-2C3A. Molecular mass markers and cleavage products of P1-2A and 2BC are indicated. m, mock-transfected extracts.