TABLE 2.

Primers used to create cleavage site mutants

Site of mutationa	Amino acid sequence of mutated siteb	Nucleotide sequence of primer pairsc
3A/3B (IPAE/G)	IPAv/G	Sense, 5′ gaa cca atc cca gct gTa ggg gta tat cat ggt gta act aag cc 3′
		Antisense, 5′ gg ctt agt tac acc atg ata tac ccc tAc agc tgg gat tgg ttc 3′
	IPAq/G	Sense, 5′ gag gaa gaa cca atc cca gct Caa ggg gta tat cat ggt gt 3′
		Antisense, 5′ ac acc atg ata tac ccc ttG agc tgg gat tgg ttc ttc ctc 3′
	ItAE/G	Sense, 5′ gag gaa gaa cca atc Aca gct gaa ggg gta tat cat ggt gt 3′
		Antisense, 5′ ac acc atg ata tac ccc ttc agc tgT gat tgg ttc ttc ctc 3′
	ItAq/G	Sense, 5′ gag gaa gaa cca atc Aca gct Caa ggg gta tat cat ggt gt 3′
		Antisense, 5′ ac acc atg ata tac ccc ttG agc tgT gat tgg ttc ttc ctc 3′
3B/3C (VESQ/S)	(VESQ/l	Sensed, 5′ gat gca gat cca gta gaa tct cag tTa act ttg gaa ata gca gga c 3′
		Antisensed, 5′ g tcc tgc tat ttc caa agt tAa ctg aga ttc tac tgg atc tgc atc 3′

^aThe wild-type amino acid sequence is shown in parentheses. 

^bMutated amino acids are shown in bold lowercase letters. 

^cMutated nucleotides are shown in bold capital letters. 

^dNew HpaI restriction site is shown in italics.