Figure 2. Comparison of c-Fos cell density, in the paraventricular nucleus of the thalamus (PVT), of fed versus fasted female mice.
(A) Experimental paradigm. Zeitgeber (ZT). (B) Percentage of body weight change at the start and end of the fasting period. (C, D, E, F, G, H, I, J, K, L, M, N, O) Representative images of c-Fos–positive cells in the anterior PVT (aPVT), mid PVT (mPVT), and posterior PVT (pPVT). (C, E, G) The red dashed outline in (C, E, G) represents regions of interest drawn around the PVT for analysis. (I, P) Quantification of c-Fos cell density in daytime (I) and overnight (P) fasting experiments. (G) Scale bars in (C, E) are 100 and 200 μm in (G). Unpaired Welch t tests: *P ≤ 0.05, ns, non-significant.
Source data are available for this figure.
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