Figure 5.

3-dimensional reconstruction of mitochondria in 1-year-old NHP offspring beta cells. (A) Reconstructed volume rendering from Imaris depicting beta cells and mitochondria labelled by an antibody cocktail directed against the electron transport chain in an islet from a 1-year-old offspring of a control diet-fed dam. White dashed circle depicts the beta cell in which the mitochondrial network is represented on the right. (B) Reconstructed volume rendering from a 1-year-old NHP offspring of a Western-style diet-fed dam. (C) Beta-cell mitochondrial volume in 1-year-old offspring. (D) Percentage of mitochondrial volume classified as filamentous (Sphericity <0.31), intermediate (Sphericity between 0.31 and 0.45), or fragmented (Sphericity greater than 0.45). C/W: dam control diet/offspring Western-style diet; W/W: dam Western-style diet/offspring Western-style diet. C/W: n=4 (2M, 2F); W/W: n=4(2M, 2F). Scale bars = 4 μm.