FIG. 7.

Inhibition of transcription and protein synthesis did not prevent the degradation of STAT1 in SV5-infected cells. 2fTGH cells were untreated (Un) or treated with actinomycin D (Act. D; 10 μg/ml) or cycloheximide (Cx; 50 μg/ml) 1 h prior to being mock infected or infected with SV5. Actinomycin D or cycloheximide (as appropriate) was also present throughout infection period. Total-cell extracts were made at 6 h p.i., and the levels of STAT1 were detected by immunoblot analysis.