Table 2. Most Relevant Fitting Parametersa; pH = 5.0 Complex Coacervation of Hydrolyzed CMS with PEO-b-PMAA for t = 0 to 10 h with a 1 h Step Interval, Final State (t = 72 h), and C3Ms at pH = 5.0 (CS).
| t (h) | P (−) (×102) | Rtot (nm) | ccolb | fcol (−) | Mw (MDa) (×106) | fw (−) |
|---|---|---|---|---|---|---|
| 0.0 | 0.0 ± 0.0 | 1.2 ± 0.4 | 0.00 | 0.00 | 0.00 ± 0.00 | 0.6 ± 0.1 |
| 1.0 | 0.4 ± 0.1 | 3.4 ± 0.1 | 0.02 | 0.00 | 0.06 ± 0.01 | 0.56 ± 0.07 |
| 2.0 | 0.5 ± 0.1 | 6.0 ± 0.2 | 0.21 | 0.00 | 0.09 ± 0.02 | 0.88 ± 0.02 |
| 3.0 | 0.7 ± 0.1 | 6.7 ± 0.3 | 0.56 | 0.00 | 0.12 ± 0.01 | 0.88 ± 0.02 |
| 4.0 | 1.5 ± 0.0 | 8.8 ± 0.2 | 0.92 | 0.00 | 0.25 ± 0.00 | 0.89 ± 0.01 |
| 5.0 | 2.1 ± 0.0 | 9.0 ± 0.0 | 1.20 | 0.00 | 0.35 ± 0.00 | 0.85 ± 0.01 |
| 6.0 | 2.9 ± 0.1 | 9.6 ± 0.1 | 1.39 | 0.00 | 0.50 ± 0.02 | 0.83 ± 0.01 |
| 7.0 | 2.9 ± 0.0 | 9.8 ± 0.0 | 1.51 | 0.07 | 0.48 ± 0.00 | 0.84 ± 0.01 |
| 8.0 | 2.9 ± 0.1 | 10.3 ± 0.1 | 1.61 | 0.09 | 0.48 ± 0.02 | 0.86 ± 0.02 |
| 9.0 | 3.3 ± 0.1 | 10.4 ± 0.2 | 1.67 | 0.10 | 0.54 ± 0.02 | 0.85 ± 0.02 |
| 10 | 4.3 ± 0.1 | 10.6 ± 0.1 | 1.71 | 0.12 | 0.71 ± 0.02 | 0.82 ± 0.02 |
| 72 | 4.8 ± 0.0 | 11.0 ± 0.1 | 1.83 | 0.14 | 0.81 ± 0.01 | 0.81 ± 0.01 |
| CS | 6.8 ± 0.1 | 13.5 ± 0.0 | 2.89 | 0.45c | 1.13 ± 0.02 | 0.88 ± 0.01 |
a
Standard deviation in the fits was determined by manual parameter changing, maximally allowing a 10% increase in Χ2, which we used as a parameter to ensure least-squares fitting.
b
ccol is the concentration (mg/mL) of colistin from CMS, calculated based on the concentration of colistin at certain time points in the kinetic model (Figure 6B).
c
Not all CS is complexed by the PEO-b-PMAA because of reduced polymer charge at pH = 5.0, and fits are made with a free fraction of CS of 45%, which was a fitting parameter.