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The ISP effects on mammalian cells are indirect. MKF were plated in 96-well tissue culture dishes (104 cells/well) and treated with serial dilutions of IFN-β, ISP preparation, or the cultured media from primary murine spleen, MEF, or RAW264.7 cells which had been treated with serial dilutions of the ISP preparation for 24 h. The MKF were washed and then treated with VSV. Cells were monitored for viability as described. Each point represents the mean of triplicate wells.
