Table 2. MICSSS marker density reported as log₂ fold change post-NACT compared to pre-NACT in tumor + tTIL + sTIL.

IHC stain	Marker of	Average	Pt 1	Pt 2	Pt 3	Pt 4	Pt 5
CD20	B lymphocytes	−1.7	−1.1	2.6	−0.2	−1.4	−5.9
CD3	T lymphocytes	−0.1	−0.6	−1.2	1	−0.7	−0.8
CD8	Cytotoxic T lymphocytes	−0.2	0.3	−0.9	0.7	−1.6	−2.5
FOXP3	Treg lymphocytes	−1.7	−1.4	0.5	−1.8	−1.4	−2.9
PD-1	Treg and T lymphocytes	0.1	0.2	0.8	1.3	−4.4	−3.8
PD-L1	Macrophages & tumor cells	−1.4	−2.2	5.3	−1.2	−3.7	−4
CD68	Monocytes/macrophages	−0.1	−0.2	−0.6	0.8	−1.3	−0.1
CD163	Monocytes/macrophages	−0.5	−1.1	−0.9	0.9	−0.6	−1.8
DC-LAMP	Mature dendritic cells	−2.8	−1.9	4.2	−2.2	−0.1	−6.5
CD8:FOXP3 ratio	–	0.4	3.2	−5.3	7.8	−0.7	0.4
Time to recurrence (days)	–	285	204	111	598	267	243

MICSSS, multiplexed immunohistochemical consecutive staining on single slide; NACT, neoadjuvant chemotherapy; TIL, tumor infiltrating lymphocyte; tTIL, tumor TIL; sTIL, stromal TIL; Pt, patient; Treg, regulatory T cell.