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Detection of the ankyrinNew Haven mutation. A: Chromatograms corresponding to exon 1 of the ankyrin gene. Compared to wild-type (black), the proband (gray) exhibits a variant chromatogram. B: Partial nucleotide sequence of exon 1 of the ankyrin gene from a PCR-amplified, subcloned fragment from the spherocytosis proband. The wild-type sequence ATG encoding codon 1 is mutated to ATA, changing the initiator methionine to isoleucine.
