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. Author manuscript; available in PMC: 2024 Aug 7.
Published in final edited form as: Am J Hematol. 2008 Oct;83(10):789–794. doi: 10.1002/ajh.21254

Figure 5.

Figure 5.

Immunoblotting of in vitro transcription/translation products using ankyrin antibodies. Products of in vitro transcription/translation experiments were analyzed by SDS-PAGE on 4–20% gels and transferred to Immobilon-P membranes. A: Incubation of a strip of this membrane containing products of wild-type plasmids with a NH2-terminal ankyrin antibody recognized a band of ~210 kDa. B: Incubation of a strip of immobilon-P membrane containing products of AnkyrinNew Haven plasmids with a NH2-terminal ankyrin antibody did not recognize any bands. C: Incubation of a strip of Immobilon-P membrane containing products of wild-type plasmids with a full length ankyrin antibody recognized a band of ~210 kDa. D: Incubation of a strip of Immobilon-P membrane containing products of AnkyrinNew Haven plasmids with full length ankyrin antibody did not recognize any bands.