Figure 6.

The expression of IDO1, RORγ, and Foxp3 in the colon and ileum. The expression of IDO1 at the transcriptional level and protein level in the (A–C) colon and (D–F) ileum of the experimental group at the 9th day post infection; RORγ and Foxp3 were detected by immunoblot in the (G–I) colon and (J–L) ileum of the experimental group at 9th day post infection. Each dot represents data from one animal. Data are mean ± SEM. n = 3. Con: Female BALB/c mice aged 6 to 7 weeks (20 ± 2 g) were treated with PBS; Inf group: Female BALB/c mice aged 6 to 7 weeks (20 ± 2 g) were treated with influenza virus infection; Ant group: Female BALB/c mice aged 6 to 7 weeks (20 ± 2 g) were treated with the combination of antibiotics. Statistical analysis was performed by independent samples T test. *P < 0.05; **P < 0.01; ***P < 0.001; ns, no significant difference. All replicates are biological.