Table 3.
Gene | Primer Sequences (5′–3′) |
---|---|
Virus matrix protein | F: GGACTGCAGCGTAGACGCTT R: CATCCTGTTGTATATGAGGCCCAT |
TLR7 | F: CTTTGGACCCCAGTAGAACAG R: GCACAGACAAGCATTTGACAG |
Myd88 | F: ACGGTCGGACACACACAAC R: TCCCACAAACAAAGGAACTG |
IFN-α | F: AAGAGGTTCAAAATCTGCTGG R: AAGAAATGAGAAGACTCCCCC |
IFN-β | F: CATCTTCTCCGTCATCTCCATA R: TGCGTTCCTGCTGTGCTT |
IDO1 | F: AGAGGATGCGTGACTTTGTG R: ATACAGCAGACCTTCTGGCA |
RORγ | F: CCGCTGAGAGGGCTTCAC R: TGCAGGAGTAGGCCACATTACA |
Foxp3 | F: GGCAATAGTTCCTTCCCAGAGTT R: GGGTCGCATATTGTGGTACTTG |
GAPDH | F: CCTCGTCCCGTAGACAAAATG R: TCTCCACTTTGCCACTGCAA |
All bacteria | F: ACTCCTACGGGAGGCAGCAGT R: ATTACCGCGGCTGCTGGC |
Enterobacteriaceae | F: GTGCCAGCMGCCGCGGTAA R: GCCTCAAGGGCACAACCTCCAAG |
Lactobacillus | F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG |
F, forward primer; R, reverse primer.