Table 3.

In vitro rumen fermentation parameters and methane concentration of whole-plant corn silage

Items1	Treatments2				SEM	P-value
	Control	MTD/1	ATCC14917	CICC24194
pH	6.65	6.58	6.51	6.50	0.020	0.093
NH3-H, mg/100 mL	19.4b	21.5b	25.6a	27.3a	0.449	0.001
IDVMD, g/kg DM	51.9c	53.3bc	55.0ab	55.8a	0.237	0.002
Total gas, mL/g	124a	116ab	110ab	109b	1.590	0.040
CH4, mL/g	12.8a	10.7b	9.15c	8.95c	0.129	< 0.001
CH4-to-total gas, % mL	10.3a	9.15b	8.28c	8.18c	0.051	< 0.001
Total VFA, mmol/L	49.8a	47.6b	47.8b	48.7ab	0.176	0.008
Acetate, mmol/L	31.9a	29.8b	29.5b	28.0c	0.139	< 0.001
Propionate, mmol/L	6.35c	7.37bc	8.5ab	10.0a	0.231	0.003
Acetate to propionate	5.05a	4.06b	3.48bc	2.81c	0.108	< 0.001
Butyrate, mmol/L	8.80a	7.88b	6.80c	6.45c	0.102	< 0.001
Isobutyrate, mmol/L	0.26	0.23	0.27	0.25	0.007	0.378
Valerate, mmol/L	0.97b	0.85b	0.88b	1.42a	0.020	< 0.001
Isovalerate, mmol/L	1.48c	1.39c	1.90b	2.47a	0.039	< 0.001

¹NH₃-H Ammonia, DM Dry matter, IDVMD In vitro DM digestibility, CH₄ Methane, VFA Volatile fatty acids

²Control, distilled water; MTD/1, MTD/1 treatment; ATCC14917, ATCC14917 treatment; CICC24194, CICC24194 treatment

³SEM Standard error of the means

^a–cMeans within the same row with different superscript letters differ (P < 0.05)