Fig 1.

Fluorescent polarization assay setup. Different additives to the buffer used for the assay were tested to find the best conditions. All indicated reagents (below the graphs) were assayed in a 50-mM phosphate buffer, pH = 8. Concentrations assayed are indicated in the Materials and Methods section and were normalized in a logarithmic scale on the x-axis. On the y-axis, polarization fluorescence is indicated in mP units. Recombinant TcBD2m was used as a negative control of binding. Grainer plates of 1,536 wells were used for all assays, and the reading was made with PheraStar Technology. Open symbols, TcBD2m; filled symbols,TcBD2WT.