FIG. 5.

NS4A/B is required in cis for the formation of p58. Cells were cotransfected with the indicated constructs. (A) Proteins were immunoprecipitated with NS5A antiserum (α-NS5A) and loaded onto an SDS–7.5% polyacrylamide gel. (B) Proteins were immunoprecipitated with NS4 antiserum (α-NS4) and loaded onto an SDS–15% polyacrylamide gel. Arrows on the right indicate HCV-specific proteins. 3/5A, pcD3/5A, 3-4A/5A, pcD3-4A/5A; 3/4B-5A, pcD3/4B-5A; 4A, pCITE4A; 4B, pCITE4B; M, molecular weight marker proteins.