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NS5A hyperphosphorylation requires an active NS3 protease. Cells were transfected with the indicated plasmids in the presence (+) or absence (−) of plasmid pcD3-4A. Proteins were immunoprecipitated with NS5A (α-5) or NS3 (α-3) antiserum and loaded onto an SDS–7.5% polyacrylamide gel. HCV-specific proteins are indicated by arrows. The positions of NS5A p56 and p58 expressed from the control plasmid pcD3-5A are indicated on the left. M, molecular weight marker proteins.
