Fig. 2.

Gene ontology process enrichment analyses of differentially expressed genes between gestational time points in CFTR^−/− sheep pancreas. Differentially expressed genes (DEGs) were filtered to enrich for genes with a fold change ≥  ± 1.5 and Benjamini–Hochberg adjusted p-value ≤ 0.01 for all samples. Gene ontology analysis by DAVID (A, C, E) or gProfiler (B) and the top 20 biological processes (BP) are shown. A, B BP from all DEGs at 80 days compared to 65 days (27 downregulated and 31 upregulated); C BP from genes (49) upregulated at term compared to 120 days; E BP from genes (246) downregulated at term compared to 120 days. D, F Volcano plots (− log₁₀ p-value against log₂ fold change) show DEGs between CFTR^−/− pancreas tissue at (D) 65 days and 80 days, and (F) 120 days and term. Genes noted in the results are marked