Fig. 3.

Gene ontology process enrichment analyses of differentially expressed genes between WT and CFTR^−/− sheep pancreas at individual gestational time points. Differentially expressed genes (DEGs) were filtered to enrich for genes with a fold change ≥  ± 1.5 and Benjamini–Hochberg adjusted p-value ≤ 0.01 for all samples. Gene Ontology analysis by gProfiler (A) or DAVID (C, D) and up to the top 20 biological processes (BP) are shown. A BP from genes downregulated at 65 days in CFTR^−/− compared to WT; C BP from all DEGs comparing WT and CFTR^−/− at 65 days; D BP from genes upregulated at 65 days in CFTR^−/− compared to WT. B Volcano plot (-log₁₀ p-value against log₂ fold change) shows DEGs between CFTR^−/− compared to WT at 65 days with a p-value ≤ 0.01 (80 genes downregulated and 52 upregulated)