Fig. 4.

Gene ontology process enrichment analyses of differentially expressed genes between WT and CFTR^−/− sheep pancreas at individual gestational time points. Differentially expressed genes (DEGs) were filtered to enrich for genes with a fold change ≥  ± 1.5 and Benjamini–Hochberg adjusted p-value ≤ 0.001 for all samples. Gene ontology analysis by DAVID (A, D) or gProfiler (B, E) and up to the top 20 biological processes (BP) are shown. A, B BP from genes (246) downregulated in CFTR^−/− compared to WT at term; D, E BP from genes (183) upregulated in CFTR^−/− compared to WT at term. C Volcano plot (− log₁₀ p-value against log₂ fold change) shows DEGs between CFTR^−/− compared to WT at term with a p-value ≤ 0.01 (936 genes downregulated and 717 upregulated)