Fig. 6.

Single-cell RNA-seq defines cell types in 80-day WT and CFTR^−/− sheep pancreas and identifies an activated stellate cell/injured Schwann cell population that is significantly overrepresented in the CFTR^−/− pancreas. UMAP plots of merged WT (AXH033, AXH066, AXH067, AXH068) and CFTR^−/− (AXH059, AXH115, AXH116) sheep pancreas by differential gene expression profiles (A) or donor genotype (B, black font WT, grey font CFTR^−/−). Twelve cell clusters are seen in (A, B) including acinar cells, endocrine cells, stromal cells, duct cells, three immune cell clusters, rapidly dividing cells, quiescent stellate cells/myofibroblasts, and activated stellate cells/injured Schwann cells (cluster 6, arrowed). Marker gene lists are shown in Table S7. C Single cell proportion test comparing CFTR^−/− with WT shows significant over representation of activated stellate cells/injured Schwann cells and duct cells, and under representation of acinar cells. D Feature plots show cluster marker genes for clusters 0–7