Fig. 7.

Single-cell RNA-seq defines cell types in 120-day WT and CFTR^−/− sheep pancreas and identifies an activated stellate cell/injured Schwann cell population that is significantly overrepresented in the CFTR^−/− pancreas. UMAP plots of merged WT (AXH039, AXH040, AXH073) and CFTR^−/− (AXH079, AXH080, AXH121, AXH122, AXH127) sheep pancreas by differential gene expression profiles (A) or donor genotype (B, black font WT, grey font CFTR^−/−). Nine cell clusters are seen in (A, B) including acinar cells, duct cells, endocrine cells, stromal cells, endothelial cells, immune cells, rapidly diving cells, quiescent stellate cells/myofibroblasts, and activated stellate cells/injured Schwann cells (cluster 8, arrowed). Marker gene lists are shown in Table S8. C Single cell proportion test comparing CFTR^−/− with WT shows significant over representation of activated stellate cells/injured Schwann cells. D Feature plots show cluster marker genes for clusters 0–7