Figure 1.

Standardized complex microbiomes selectively affect male asd-related behavior.

(a) Graphical representation of experimental design depicting cohorts of neonatal (n = 10-12 mice/sex/GM) and adult (n = 20 mice/sex/GM) BTBR mice.

(b) Dot plot depicting Chao-1 Index. ***p_GM < 0.001, p_Sex = 0.002, Two-way ANOVA

(c) Dot plot depicting Shannon Index. p_GM = 0.867, p_Sex = 0.276, Two-way ANVOA

(d) Principal coordinate analysis depicting Bray-Curtis dissimilarity between microbial communities. p_GM < 0.001, p_Sex = 0.063, Two-way PERMANOVA.

(e) Dot plot depicting USV rate. *p < .05, **p < .01, Tukey post hoc.

(f) Principal coordinate analysis depicting Bray-Curtis dissimilarity of the relative abundance of ultrasonic vocalizations. p_GM = 0.001, p_Sex = 0.044, Two-way PERMANOVA.

(g) Stacked bar charts depicting mean relative abundance of call types determined by VocalMat.

(h) Dot plot depicting Grooming Index. p_GM = 0.321, p_Sex = 0.069, Two-way ANVOA.

(i) Dot plot depicting Burying Index. p_GM = 0.862 p_Sex = 0.048, Two-way ANOVA.

(j) Dot plot depicting time spent in Stranger (closed circles) or Object (open circles) chambers of social preference test.

(k) Tukey box plot depicting Social Preference Index. *p_GM = 0.044, p_Sex = 0.498, Two-way ANOVA.