Table 3.
Primers used in this study.
| Primer name | Sequence (5’-3’) | Description |
|---|---|---|
| 0190XbaI-Fw | TGCATCTCTAGATTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCGAAAAGACCGTGACAACGG | To amplify fragment containing pscAMT |
| 0192Pst1-Rv | TGCCTCCTGCAGGACGGTGTTCAGATTAAGTG | To amplify fragment containing pscAMT |
| 183 BamHI Fw | TGCATCGGATCCAGGAGGCACTCACGACGATAATGACAATAC | To amplify fragment containing pscEFG, pscEFGHI and pscEFGHIKR |
| 185 MluI Rv | TGCCTCACGCGTCTTTCCATGGTGTTGGGTG | To amplify fragment containing pscEFG |
| 187 MluI Rv | TGCCTCACGCGTGATTCTCCTTCCCTGGGT | To amplify pscEFGHI fragment |
| 189 MluI Rv | TGCCTCACGCGTCACGTGAGAAAATACCGGTC | To amplify fragment containing pscEFGHIKR |
| 186 XbaI Fw | TGCATCTCTAGAAGGAGGCACTCACGGCTTCATCATGCACA | To amplify fragment containing pscH and pscHI |
| 186 HindIII Rv | TGCCTCAAGCTTGGTCATTGCTCCTTGGAT | To amplify fragment containing pscH |
| 187 XbaI Fw | TGCATCTCTAGAAGGAGGCACTCACCCATGGCAAACACATC | To amplify fragment containing pscI |
| 187 HindIII Rv | TGCCTCAAGCTTCGTTGTCAGTCGGATT | To amplify fragment containing pscI and pscHI |
| Intern 190–191 Fw | GACAACGGTGGCTCCTCCTGC | Confirmation of recombinant in B. breve UCC2003 |
| Intern 190–191 Rv | CCAGACAAGCTTCCTCTGAC | Confirmation of recombinant in B. breve UCC2003 |
| pPTPi_Fw | TGATTTCGTTCGAAGGAACTA | Multicloning site pPTPi |
| pPTpi_Rv | TGGCGGACAATAAGTCCTC | Multicloning site pPTPi |
Restriction sites incorporated into oligonucleotide primer sequences are indicated in bold and promoter regions incorporated into nucleotide primer sequences are indicated in italics.