Table 3.
List of gene primer sequences.
| Gene | GenBank ID | PCR primers sequence (5′-3′) | PCR products(bp) |
|---|---|---|---|
| ACC | NM_205505.2 | F-ACTCCATCGCATCTTCCATTATGTC R-TCAGTGTCTTCATTAGTCGCTCAAC |
125 |
| HMGR | NM_204485.3 | F-GAAGCAGTCATTCCAGCCAAGG R-AGCCATAGCAGAACCCACCAG |
125 |
| ATGL | NM_001113291.2 | F-TTCTGACAACTTGCCACGATATGAG R-TTGGATGCTTGTATTGGTGACTCTC |
105 |
| CPT1A | NM_001012898.1 | F-GAGTCAGACACCACAGCAACAC R-CCGTAACCATCATCAGCCACAG |
105 |
| SREBP-1C | NM_204126.3 | F-AAGGCGGAGGTGATGGAGATG R-GGCTGTCAGGCTCGGAGTC |
133 |
| AMPKα | NM_001039603.2 | F-CCATCTGTCTCGCCCTCATCC R-AATGCCACTTCGCTCTTCTTACAC |
133 |
| β-actin | NM_205518.2 | F-CAGCCATGTATGTAGCCATCCAG R-GTAACACCATCACCAGAGTCCATC |
96 |
Abbreviations: ACC, Acetyl-CoA carboxylase; HMGR, 3-hydroxy-3-methyl glutaryl coenzyme A reductase; ATGL, Adipose triglyceride lipase; CPT1A, Carnitine palmitoyltransferase1; SREBP-1C, sterol regulatory element-binding protein-1c; AMPKα, Adenosine 5′-monophosphate (AMP)-activated protein kinase.