Fig. 3.

Effect of Nurr1 overexpression on neuropathological changes in the internal capsule and the striatum after ICH induction. a Left, a low magnification image of GFP fluorescence at 4 weeks after AAV-GFP injection. Cx: cerebral cortex, IC: internal capsule, H: hematoma. Middle and right, representative images of GFP fluorescence in the internal capsule at 3 ​d after ICH induction. b Quantitative results of morphological changes of GFP-positive axonal fibers after ICH induction. $$ P ​< ​0.01. c Representative images of neurofilament-H immunofluorescence in the internal capsule at 3 ​d after ICH induction. d Quantitative results of morphological changes of neurofilament-H-immunopositive axonal fibers after ICH induction. $ P ​< ​0.05. e Top, schematic representation of the area examined for Iba1 immunofluorescence. Middle, representative images of Iba1-immunopositive signals in peri-hematoma region at 3 ​d after ICH induction. Bottom, quantitative results on the number of Iba1-positive cells. f Top, schematic representation of the area examined for GFAP immunofluorescence. Middle, representative images of GFAP-immunopositive signals in peri-hematoma region at 3 ​d after ICH induction. Bottom, quantitative results on GFAP-positive area. g Top, schematic representation of the area examined for nitrotyrosine immunofluorescence. Middle, representative images of nitrotyrosine-immunopositive signals at 3 ​d after ICH induction. Broken lines indicate the edge of hematoma. Bottom, quantitative results on nitrotyrosine-positive area. h Top, schematic representation of the area examined for NeuN immunofluorescence. Middle, representative images of NeuN-immunopositive signals in the central region of the hematoma for at 3 ​d after ICH induction. Bottom, quantitative results on the number of NeuN-positive cells. n.s., not significant. Data were statistically analyzed by unpaired t-test. Number of mice examined was 7 in AAV-GFP ​+ ​ICH group and 8 in AAV-GFP-Nurr1 ​+ ​ICH group, respectively.