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. 2024 Aug 7;10(32):eadn7187. doi: 10.1126/sciadv.adn7187

Fig. 6. TLRa-SNP incorporating both TLR4a and TLR7/8a generated synergistic effect.

Fig. 6.

(A) Schematic representation of TLR4a-TLR7/8a-SNP for which SNPs coincorporate TLR4a MPLA and TLR7/8a imidazoquinoline derivative. (B) Timeline of RBD-NP vaccines immunization and blood collection to determine IgG titers. Mice were immunized on week 0 and boosted on week 3 with RBD-NP vaccines adjuvanted with a 1:1 mixture of TLR4a-SNP and TLR7/8a-SNP or TLR4a-TLR7/8a-SNP. IgG1, IgG2c, and variants titers were measured on week 5. (C) Anti-RBD IgG binding endpoint titers of RBD-NP vaccines. (D) IgG1 titers and (E) IgG2c titers. (F) Ratio of anti-RBD IgG2c to IgG1 titers. Lower values (below 1) suggest a TH2 response or humoral response, and higher values (above 1) imply a TH1 response or cellular response. (G) Week 7 competitive binding curves for different RBD-NP vaccines compared to an mAb reference competing with the same mAb. OD, optical density. (H) Calculated KD values from fitted binding curves. Data (n = 4 to 5) are shown as means ± SEM. P values were determined using the GLM followed by Tukey’s post hoc comparison procedure on the logged titer values for IgG titer comparisons. Complete P values for comparisons are shown in tables S19 to S22. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.