Table 2. PCR conditions for gRNA library amplification.

Temperature	Time	Cycles
PCR1
98°C	3 min	–
98°C	30 s	× 20
56.5°C	20 s
72°C	60 s
72°C	2 min	–
4°C	Hold	–
PCR2
98°C	3 min	–
98°C	30 s	× 8
56.5°C	20 s
72°C	60 s
72°C	2 min	–
4°C	Hold	–