TABLE 3.
Relative resistance of DF-1 cells expressing sTva-mIgG to ALV(A) infection
| Vector | RCASBP(A)AP titer (mean ± SD) | Resistancea | Concn of sTva-mIgG (ng/ml)b |
|---|---|---|---|
| Uninfected DF-1 | (3.1 ± 0.1) × 106 | ||
| RCASBP(C) | (1.2 ± 0.1) × 106 | 2.6 | |
| RCASBP(C)stva-mIgG | (5.4 ± 0.2) × 103 | 574 (221) | 1714 ± 97 (48 nM) |
| RCAS(C) | (6.2 ± 0.2) × 105 | 5 | |
| RCAS(C)stva-mIgG | (5.7 ± 0.4) × 103 | 544 (109) | 789 ± 26 (22 nM) |
| RCOSBP(C) | (9.0 ± 0.9) × 105 | 3.4 | |
| RCOSBP(C)stva-mIgG | (5.2 ± 0.8) × 104 | 60 (18) | 394 ± 26 (11 nM) |
a
The resistance of the cells to ALV(A) infection was determined by dividing the mean titer obtained on the control uninfected DF-1 cells by the mean titer obtained for each experimental group. The resistance of the cells to ALV(A) infection relative to the vector alone is given in parentheses.
b
Concentration of sTva-mIgG protein in the supernatants as quantitated by ELISA for the mIgG tag. The molar concentration of the sTva-mIgG protein is given in parentheses. The apparent molecular weight of sTva-mIgG was calculated from the amino acid sequence and found to be 35,911.