Figure 2.

Excitatory currents onto DRN serotonergic neurons are primarily mediated by AMPAR. A: schematic of the experiment illustrating the placement of the bipolar-stimulating electrode in the DRN and recording of evoked excitatory currents from 5-HT neurons. B: representative traces of electrically evoked excitatory synaptic currents recorded from 5-HT neurons at holding potentials of −70 mV and +40 mV. Recordings at +40 mV were performed in the presence of the AMPA antagonist NBQX (10 µM). C: quantification of AMPA/NMDA ratios across maturation. P6, n = 11 cells (6 mice); P15, n = 9 cells (6 mice); P21–23, n = 10 cells (9 mice); P33–37, n = 10 cells (7 mice); P45–47, n = 12 cells (8 mice); P60–80, n = 16 cells (11 mice). Bar graphs represent means ± SE. **P < 0.01, one-way ANOVA followed by Tukey’s multiple comparison test. DRN, dorsal raphe nucleus; NMDA, N-methyl-d-aspartate.