FIG. 3.

FACS analysis of H-2 haplotype in wild-derived mouse strains. Splenocytes from BR, PE, and CZ mice were stained with the FITC-labeled anti-MHC class I (H-2^k) MAb. A monoclonal mouse IgG1 was used as a negative control. Spleen cells of BR mice served as a positive control. The analysis was performed by using a flow cytometer after gating on the viable cell population as determined by forward- and side-scatter analysis. Positive staining and fluorescence intensity (log scale) for 10,000 events are shown. The histograms represent one of three experiments. Similar results were obtained by using anti-class II (H-2^k) MAb. The levels of surface positive cells were <1% with anti-H-2^b class I MAb compared to >90% with anti-H-2^k MAb (BR and PE mice).