TABLE 2.

Specificities of LN-13.1 CTL cell line

Type of specificity and antibody or peptide used	% Specific lysis
MHC restriction specificitya
mIgG	35
Anti-H-2Kk	25
Anti-H-2Dk	0
Peptide specificityb
mT-E328	11
mT-R389	92

^a Tumor cells (A-6215) derived from an irradiated, virus-infected BR mouse were used as targets. These cells were pretreated with either anti-H-2K^k or anti-H-2D^k MAbs (5 μg/well). A monoclonal mouse Ig6 (mIgG; specific for TNP) was used as a control. 

^b Plastic-adherent cells from naïve BR spleens were pulsed with middle-T peptides, and the CTL assay was carried out at a 5:1 effector-to-target ratio.