TABLE 6.
Mixed lymphocyte reactionsa
| Stimulator lymphocyte group | [3H]thymidine uptake (cpm)
|
|
|---|---|---|
| Expt 1 | Expt 2 | |
| Medium | 2,228 | 2,191 |
| PE | 1,052 | 1,776 |
| CZ | 28,523 | 28,749 |
| BR | 950 | 931 |
| B6 | 37,304 | 37,882 |
a
Stimulator lymphocytes from spleens of different mouse strains were treated with mitomycin C. Responder cells were splenocytes from BR mice throughout. Reactions were performed in 96-well plates containing 4 × 105 stimulator lymphocytes plus 2 × 105 responder lymphocytes per well. The cells were incubated for 4 days and then for an additional 16 h in the presence of 1 μCi of [3H]thymidine per well. Thymidine incorporation was measured on a beta scintillation counter. Values are the means of triplicate cultures.