Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jul 12;9(8):2099–2112. doi: 10.1038/s41564-024-01755-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a, Experimental setup of intranasal inoculation of Syrian hamsters (Mesocricetus auratus, male, 12 weeks old (WT, OTS4-5 and OTS7-8) and 8 weeks old (OTS-206) (Donors) (n=10 hamsters/OTS-group until 5dpi, n=5 hamsters/OTS-group until 21dpi, n=4 hamster WT-group until 5dpi) with OTS4-5, OTS7-8, or OTS-206 SARS-CoV-2, including co-housing of naïve contact animals (Contacts) (n=4 hamsters/group until 21dpi) 1 day post inoculation (dpi). Data obtained from two independent experiments (experiment 1 (OTS4-5, OTS7-8, WT) and experiment 2 (OTS-206). Overview created with BioRender.com. b, c, Body weight changes of inoculated and contact hamsters in percent as mean data (+/- SEM) and on individual level. d, e, Virus genome copy numbers in nasal washings of donor and contact hamsters. f, g, Virus genome copy numbers in the organ samples of donors at 5 and 21 dpi. h, Virus genome copy numbers in the organ samples of contact hamsters at 21 dpi. i, Serum samples of 5 and 21 dpi analyzed by SARS-CoV-2RBD-ELISA. j, Serum samples were additionally analyzed in live virus neutralization assay (capacity to neutralize 100 TCID50) against ancestral WT SARS-CoV-2. k, Pneumonia-induced pulmonary atelectasis 5 dpi given in % affected area. l, Histopathology, lung whole slide images showing atelectasis, hematoxylin-eosin stain. Scale bar, 2.5 mm. m, Virus antigen score, 0= no antigen, 1 = focal, 2 = multifocal, 3 = coalescing, 4 = diffuse. n, Representative virus antigen imaging (WT group) by immunohistochemistry (SARS-CoV nucleocapsid protein detection), mainly in type-1 pneumocytes, bar 100 µm. Statistical significance was assessed by two-sided, unpaired, nonparametric multiple t-test with Mann-Whitney test (compared ranks, CI: 95%) (panel b-h). *P<0.05, **P < 0.01, ***P<0.001, ****P<0.0001. No asterisk indicates no statistical significance.

Source data