Extended Data Fig. 10. Sh-RNA-Mccn3 Knockdown Does Not Impair the Fertility or Milk Provision of Female Mothers, But Limits Pup Growth.
a, Representative images of brain sections and CCN3 staining quantification from dams injected with shControl (upper) or shRNA-mCcn3 (lower) and collected at 12DPP. Yellow border defines area in which CCN3 immunostaining intensity was quantified. Scale bars = 500 µm. b, Percent reduction in ARC CCN3 immunostaining intensity of shRNA-mCcn3 dams fed SD (N = 9) or LCD (N = 2) as compared to shRNA control dams fed SD (N = 5). c, The time interval between mating (day 0) and observation of copulatory plug (Control N = 5, shRNA-mCcn3 N = 9). d, Milk consumption in litters from shCtrl (N = 3) and shCcn3 (N = 9) as measured by weight recovery following 3-hour separation from lactating dams fed SD. e, Mean body weights for litters (N = 4 litters, N = 6 pups/litter) nursed by shCtrl mothers at 4, 8, and 12DPP (grey) or switched to an shCcn3 mother beginning at 8DPP (blue and grey). Unpaired 2-tailed Student’s T-test in panel c. Two-way ANOVA with repeated measures (Holm-Šidák’s multiple-comparisons test) in panel d. Error Bars ± SEM.