FIG. 2.

Effect of protein synthesis inhibitors on the poliovirus RNA replication cycle. Preinitiation RNA replication complexes were resuspended in reaction mixtures containing fresh HeLa S10 extract, translation initiation factors, and 15 μCi of [α-³²P]CTP (method 3 in Materials and Methods). The individual reactions also contained 2 mM guanidine (lane 1), no additional drug (lane 2), 200 μg of cycloheximide per ml (lane 3), 8 μg of anisomycin per ml (lane 4), 40 μg of diphtheria toxin per ml plus 40 μg of NAD⁺ per ml (lane 5), or 100 μg of puromycin per ml (lane 6). The reactions were incubated at 30°C for 90 min, and the labeled RNA products were characterized by CH₃HgOH-agarose gel electrophoresis and autoradiography.