Figure 3.

Concentration-related effect of AWME3 on the deposition of extracellular nucleic acids (eNAs) in K pneumoniae biofilms. Fluorescence microscopy representative images of (A) untreated cell (control) biofilm established by KP ATCC BAA-2473 strain and the same bacteria treated with (B) 0.5 MIBC (0.25 mg/ml), (C) MIBC (0.5 mg/ml), and (D) 2 MIC (1.0 mg/ml) of AWME3. PI staining used to stain eNAs of the biofilm. Relative fluorescence intensity of biofilm structures of the KP ATCC BAA-2473 strain (E) is reported in arbitrary unit (au) obtained after quantification of digital images using the Fiji Image J software. Data are expressed as the mean ± STD. ****p < 0.0001 was significant compared to the control group.