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Western blotting was used to determine the NOD2 and Beclin-1protein levels (A-B, D). The expression of relative NOD2 mRNA and Beclin-1 mRNA were detected in different groups (C, E). Data were expressed as mean ± SD, n = 6; ns: no significance; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. a: control, b: MDP, c: MDP + IPEN, d: MDP + SPEN, e: MDP + NOD2 antagonist
