TABLE 3.

Mutations that occurred during persistence of MP1 in muscle or in PI-G8 myoblasts

Genomic region (nucleotides)a	Mutations detected in persistent viral RNAb
	MP1/M		MP1/G8
	REF	Sequencing	REF	Sequencing
21–583	−	−	+	269 (C-U)
475–2133	−	NS	−	NS
2121–3993	−	NS	+	NS
3339–4392	−	NS	−	NS
4388–5523	−	NS	+	NS
5508–6668	+	6249 (GCA-GCRc) (Ala-Ala)	+	5787 (GGU-GGC) (Gly-Gly)
6280–7388	−	NS	+	NS

^aNucleotide numbers indicate the genomic region screened and exclude the primer-binding region of the amplicon. The numbering system is based on alignment of partial-sequence information for MP1 with the complete sequence of CVB1 published for the pNI4 clone. 

^bResults of REF analysis indicate whether altered migration either was (+) or was not (−) observed for restriction fragments within a given genomic region. Summarized sequence information indicates that there were no sequence changes detected within a region (−) or that the region was not sequenced (NS). Mutations are reported as the nucleotide position of the mutation followed by the base change and the codon change for coding sequences. 

^cR = A or G.