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The adr mutant I97L core protein alone is necessary and sufficient to result in the immature secretion of Dane particles. The adr wt I97I and adr mutant I97L and I97F core proteins were supplied in trans to core-defective mutant 1903 via cotransfection into human hepatoma Huh7 cells. The experimental procedures for the gradient centrifugation analysis were as described for Fig. 2.
