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Optimization of assay conditions for RNA synthesis by the BVDV NS5B. The C-terminally truncated NS5B, NS5BΔCT24-His (50 nM), was used in the optimization experiments. Poly(C) was the template, and oligo(G) was used as a primer. (A) Effect of temperatures; (B) effect of pH; (C) effect of glycerol; (D) effect of a zwitterionic detergent, CHAPS.
