FIG. 8.

Functional interaction of T-antigen with YB-1 deletion mutants. (A and B) Transcriptional activity of deletion mutant YB-1 (1–125), in the presence or absence of T-antigen (T-Ag), on CAT reporter plasmids (7.5 μg) containing the viral late (A) and early (B) promoters. The concentrations of expression plasmids are indicated at the bottom of the respective panels (in micrograms per plate). Representative CAT data are shown above the graphs, and quantitative analysis of results is expressed as CAT activity relative to the basal-level expression of the promoter. (C and D) Transcriptional activity of deletion mutant YB-1 (1–37), in the presence or absence of T-antigen, on CAT reporter plasmids (7.5 μg) containing late (C) and early (D) promoters. The experimental design was virtually identical to that described for panels A and B. The results shown in each panel represent the means of data from three independent experiments. Bars indicate standard deviations.