Table 1.
Characteristics of HPV-associated and HPV-independent head and neck squamous cell carcinomas
| Topography (case#) | p16 | HPV ISH | p53 IHC | p53 pattern | TP53 status | TP53 mutation(s) | Final classification |
|---|---|---|---|---|---|---|---|
| Oropharynx (1) | Subclonal | Pos | Wildtype | Wildtype | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Wildtype | NA | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Null-like | NA | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Basal sparing | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Basal sparing/clusters | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Basal sparing | no TP53 mut | HPV-associated | |
| Oropharynx (4) | p16 pos | Pos | Wildtype | Basal sparing | TP53 mut | D281E | HPV-associated |
| Oropharynx | p16 pos | NA | Wildtype | Null-like | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | NA | Wildtype | Basal sparing | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Clusters | no TP53 mut | HPV-associated | |
| Oropharynx | p16 pos | Pos | Wildtype | Basal sparing | no TP53 mut | HPV-associated | |
| Oral cavity | p16 pos | Pos | Wildtype | Clusters | no TP53 mut | HPV-associated | |
| Oropharynx | p16 neg | Neg | Abnormal | Weak cytoplasmic | TP53 mut | P316fs*16 | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Null | TP53 mut | L257fs*90 | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Null | TP53 mut | K139fs*25 | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Null | TP53 mut | L257fs*88, | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Null | TP53 mut | Q136* | HPV-independent |
| Oropharynx (2) | p16 neg | Neg | Abnormal | Subclonal null | TP53 mut | K120R, T155fs*19 | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | S127Y, E258K | HPV-independent |
| Oropharynx | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | C242R | HPV-independent |
| Oropharynx | p16 neg | NA | Abnormal | Overexpression | TP53 mut | R248Q | HPV-independent |
| Oropharynx | p16 neg | NA | Abnormal | Overexpression | TP53 mut | R249S | HPV-independent |
| Oral cavity | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | H193N | HPV-independent |
| Oral cavity | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | G279E | HPV-independent |
| Oral cavity | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | R273C | HPV-independent |
| Larynx, hypopharynx | p16 neg | NA | Abnormal | Null | TP53 mut | F212fs*3, splice site 673-1G > A | HPV-independent |
| Larynx, hypopharynx (3) | Equivocal | Neg | Abnormal | Overexpression | TP53 mut | Y205C | HPV-independent |
| Larynx, hypopharynx | p16 neg | NA | Abnormal | Overexpression | TP53 mut | S241F | HPV-independent |
| Larynx, hypopharynx | p16 neg | Neg | Abnormal | Overexpression | TP53 mut | E285K, C242S | HPV-independent |
| Larynx, hypopharynx | p16 neg | NA | Abnormal | Overexpression | TP53 mut | R273H | HPV-independent |
| Larynx, hypopharynx | p16 neg | NA | Abnormal | Overexpression | TP53 mut | Y205C | HPV-independent |
mut = mutation, HPV = human papilloma virus, ISH = in situ hybridization, neg = negative, pos = positive, NA = not assessed