Table 2.
Sequence of primers for tight junction proteins in jejunum of nursery pigs
| Gene1 | Primer sequences (5ʹ to 3ʹ)2 | Accession number |
|---|---|---|
| ZO-1 | F: CAGAGACCAAGAGCCGTCC | XM_003480423.4 |
| R: TGCTTCAAGACATGGTTGGC | ||
| OC | F: TCAGGTGCACCCTCCAGATT | NM_001163647.2 |
| R: AGGAGGTGGACTTTCAAGAGG | ||
| CL-1 | F: ATTTCAGGTCTGGCTATCTTAGTTGC | NM_001244539.1 |
| R: AGGGCCTTGGTGTTGGGTAA | ||
| GAPDH | F: TCGGAGTGAACGGATTTGGC | NM_001206359.1 |
| R: TGCCGTGGGTGGAATCATAC | ||
| MUC-1 | F: GGGGGACCGGTATAAAGCAG | XM_020945387.1 |
| R: CCCTTGTCATGATGGCGGTA | ||
| MUC-2 | F: CAACGGCCTCTCCTTCTCTGT | XM_021082584.1 |
| R: GCCACACTGGCCCTTTGT |
1ZO-1, zonula occludens; OC, occludin; CL-1, claudin-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MUC-1, mucin-1; MUC-2, mucin-2.
2F, forward; R, reverse.